6J1Y
Semi-open conformation E3 ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.019, 59.370, 85.075 |
| Unit cell angles | 99.39, 92.29, 108.75 |
Refinement procedure
| Resolution | 42.572 - 2.550 |
| R-factor | 0.2104 |
| Rwork | 0.208 |
| R-free | 0.25430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xmc |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.935 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.108 | 0.800 |
| Number of reflections | 33955 | 3493 |
| <I/σ(I)> | 32.6 | 2.3 |
| Completeness [%] | 95.2 | 92.3 |
| Redundancy | 4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1M Sodium malonate pH 5.0, 12% w/v Polyethyleneglycol 3350 |
