6J1X
WWP1 close conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-04-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.309, 45.444, 116.545 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 35.810 - 2.300 |
| R-factor | 0.2221 |
| Rwork | 0.220 |
| R-free | 0.27050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xmc |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.830 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.290 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 26023 | 2599 |
| <I/σ(I)> | 16.3 | 3 |
| Completeness [%] | 97.7 | 97.7 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 100mM Tris-HCl pH 7.0, 15% v/v reagent alcohol |
