6J1U
influenza virus nucleoprotein with a specific inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 119.592, 126.747, 194.425 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.530 - 2.800 |
| R-factor | 0.19499 |
| Rwork | 0.191 |
| R-free | 0.25971 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iqh |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.589 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.118 | 0.040 |
| Number of reflections | 39939 | 3247 |
| <I/σ(I)> | 13.9 | 2.6 |
| Completeness [%] | 92.3 | 94.6 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M sodium acetate, 0.05 M magnesium acetate, 0.1 M MES, pH 6.0, 7% PEG8000 and 5% glycerol |
