6IZQ
PRMT4 bound with a bicyclic compound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.788, 98.926, 206.792 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 89.240 - 2.449 |
| R-factor | 0.1925 |
| Rwork | 0.191 |
| R-free | 0.24810 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 89.240 | 2.500 |
| High resolution limit [Å] | 2.449 | 2.449 |
| Number of reflections | 56632 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 98.7 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 298 | 22-27% PEG 3350, 0.15M sodium malate, pH 7.0 |
