6IZ4

Crystal Structure Analysis of TRIC counter-ion channels in calcium release

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-17
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9778
Spacegroup name	P 42 21 2
Unit cell lengths	290.246, 290.246, 195.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.932 - 3.098
R-factor	0.2834
Rwork	0.283
R-free	0.30050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iz5
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHENIX
Refinement software	PHENIX (1.14rc2_3191)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.932	49.930	3.150
High resolution limit [Å]	3.098	16.970	3.100
Rmerge	0.389	0.040	4.774
Rmeas	0.394	0.040	4.841
Rpim	0.065	0.008	0.792
Number of reflections	137600	1028	7391
<I/σ(I)>	12
Completeness [%]	91.0	95.8	99.5
Redundancy	35.9	27.6	36.6
CC(1/2)	0.999	0.999	0.463

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	22% PEG 400, 50mM MgCl2, 100mM Glycine pH 9.0