6IYY
Crystal structure of human WIPI3,loop deletion mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9785 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 46.297, 104.931, 124.882 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.357 - 1.796 |
R-factor | 0.1681 |
Rwork | 0.165 |
R-free | 0.20410 |
Structure solution method | SAD |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.12_2829: ???) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.840 |
High resolution limit [Å] | 1.796 | 1.800 |
Rmerge | 0.065 | 0.230 |
Number of reflections | 28765 | 1811 |
<I/σ(I)> | 10.2 | 3 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 6.5 | 2.7 |
CC(1/2) | 0.990 | 0.990 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7 | 289 | 1.6M Ammonium Sulfate, 0.1M Tris-HCl |