6ISF
Structure of 9N-I DNA polymerase incorporation with dT in the active site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2017-02-22 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.9998 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 92.088, 107.361, 255.665 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.380 - 2.800 |
R-factor | 0.23363 |
Rwork | 0.232 |
R-free | 0.27162 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4k8x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.334 |
Data reduction software | XDS (November 1, 2016 built 20161205) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.380 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.074 | 0.381 |
Number of reflections | 62970 | 6160 |
<I/σ(I)> | 2.93 | |
Completeness [%] | 99.5 | |
Redundancy | 2 | |
CC(1/2) | 0.988 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Sodium Acetate pH4.6, MPD, Glycerol, CaCl2 |