6ISF

Structure of 9N-I DNA polymerase incorporation with dT in the active site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DNA polymerase	polymer	783	90939.8	2	UniProt (Q56366) Pfam (PF03104) Pfam (PF00136)	Thermococcus sp. 9oN-7
2	C, E (C, E)	DNA (5'-D(P*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*CP*CP*T)-3')	polymer	15	4561.0	2		synthetic construct
3	D, F (D, F)	DNA (5'-D(*AP*CP*AP*GP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3')	polymer	18	5550.6	2		synthetic construct
4	G, H, I, J, K... (A, B)	CALCIUM ION	non-polymer		40.1	7	Chemie (CA)
5	N, O, P (A, B, E)	water	water		18.0	48	Chemie (HOH)

Sequence modifications

A, B: 1 - 775 (UniProt: Q56366)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation
Leu 485	Ala 485	engineered mutation
Leu 776	-	expression tag
Glu 777	-	expression tag
His 778	-	expression tag
His 779	-	expression tag
His 780	-	expression tag
His 781	-	expression tag
His 782	-	expression tag
His 783	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	202102.8
Non-Polymers*	Number of molecules	7
	Total formula weight	280.5
All*	Total formula weight	202383.3

*Water molecules are not included.