6IQS

Crystal structure of Prc with L245A and L340G mutations in complex with NlpI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-27
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.10000
Spacegroup name	P 21 21 21
Unit cell lengths	119.342, 144.415, 152.504
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.910 - 2.690
R-factor	0.20695
Rwork	0.205
R-free	0.24522
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wql
RMSD bond length	0.016
RMSD bond angle	2.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000 (v712)
Phasing software	PHASER (2.70)
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.690	2.690
Rmerge	0.064	0.548
Rmeas	0.098	0.813
Rpim	0.042	0.394
Number of reflections	69840	6187
<I/σ(I)>	13.47	3.684
Completeness [%]	94.7	85.1
Redundancy	5	3.5
CC(1/2)	0.998	0.783

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	PEG3350, Sodium citrate tribasic dihydrate