6IPU
Human nucleosome core particle containing 145 bp of DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 98.15 |
| Detector technology | PIXEL |
| Collection date | 2017-09-18 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.560, 109.710, 183.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.160 - 1.990 |
| R-factor | 0.237 |
| Rwork | 0.236 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nzd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.474 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.160 | 2.100 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rpim | 0.021 | 0.652 |
| Number of reflections | 148453 | 21030 |
| <I/σ(I)> | 17.4 | 1.3 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 12.2 | 11.6 |
| CC(1/2) | 0.997 | 0.526 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | manganese chloride, potassium chloride, potassium cacodylate |
