6ILD

Crystal Structure of Human LysRS: P38/AIMP2 Complex II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-06
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	96.511, 100.074, 270.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.420 - 1.882
R-factor	0.155
Rwork	0.153
R-free	0.18440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bju
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.950
High resolution limit [Å]	1.880	4.050	1.880
Rmerge	0.068	0.026	0.571
Number of reflections	104488	10704	10197
<I/σ(I)>	9.2
Completeness [%]	98.5	97.2	97.6
Redundancy	7.9	7.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	8 % PEG 20000, 0.1 M Tris pH 8.0, 0.1 M sodium chloride