6IIK
USP14 catalytic domain with IU1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-09-01 |
| Detector | OXFORD SAPPHIRE CCD |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.869, 81.670, 107.371 |
| Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
| Resolution | 29.217 - 1.970 |
| R-factor | 0.19 |
| Rwork | 0.189 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (phenix.refine: 1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 68318 | 6105 |
| <I/σ(I)> | 28.25 | |
| Completeness [%] | 96.7 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | NH4F. PEG 3350, glycine, Cscl |
