6IID
Human EXOG-H140A in complex with RNA-DNA chimeric duplex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2018-08-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.710, 75.953, 81.152 |
| Unit cell angles | 72.96, 90.01, 70.75 |
Refinement procedure
| Resolution | 29.834 - 2.986 |
| R-factor | 0.2021 |
| Rwork | 0.199 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zki |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 2.986 | 3.000 |
| Rmerge | 0.085 | 0.432 |
| Rmeas | 0.120 | 0.611 |
| Rpim | 0.085 | 0.432 |
| Number of reflections | 30771 | 2246 |
| <I/σ(I)> | 8.9 | 1.59 |
| Completeness [%] | 96.7 | 91.9 |
| Redundancy | 1.9 | 1.8 |
| CC(1/2) | 0.941 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M magnesium formate dehydrate and 20% [w/v] PEG 3350 |
