6IF2

Complex structure of Rab35 and its effector RUSC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	P 65
Unit cell lengths	188.213, 188.213, 37.185
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.053 - 2.400
R-factor	0.1691
Rwork	0.167
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3tw8 4giw
RMSD bond length	0.008
RMSD bond angle	1.134
Data reduction software	d*TREK
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.145	0.042	0.761
Rmeas	0.153	0.044	0.805
Rpim	0.048	0.014	0.259
Total number of observations	299834
Number of reflections	30173	1632	1498
<I/σ(I)>	3.9
Completeness [%]	99.9	100	100
Redundancy	9.9	9.4	9.5
CC(1/2)		0.999	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	HEPES, PEG 3350