6IEU
The structure of TRIM66 PHD-Bromo domain with unmodified H3 N terminal peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 100.266, 63.465, 33.303 |
| Unit cell angles | 90.00, 101.67, 90.00 |
Refinement procedure
| Resolution | 32.614 - 1.787 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o33 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.886 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.614 | 1.851 |
| High resolution limit [Å] | 1.787 | 1.787 |
| Rmerge | 0.116 | 0.640 |
| Number of reflections | 19103 | 1684 |
| <I/σ(I)> | 22.409 | |
| Completeness [%] | 98.3 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES sodium, pH 7.5,10% v/v 2-propanol, 20% PEG4000 |
