6IEG
Crystal structure of human MTR4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-07 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.316, 119.139, 124.567 |
| Unit cell angles | 90.00, 99.87, 90.00 |
Refinement procedure
| Resolution | 46.950 - 3.550 |
| R-factor | 0.27 |
| Rwork | 0.268 |
| R-free | 0.30500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u4c |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.820 |
| High resolution limit [Å] | 3.546 | 3.546 |
| Rpim | 0.110 | 0.347 |
| Number of reflections | 29840 | 5874 |
| <I/σ(I)> | 6.25 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 15 mM Tricine pH 8.5, 12% (w/v) PEG 4000 |
