6IBD
The Phosphatase and C2 domains of Human SHIP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.889, 80.076, 90.273 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.900 - 1.480 |
| R-factor | 0.17385 |
| Rwork | 0.173 |
| R-free | 0.20054 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5okm |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.791 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.080 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.187 | 3.948 |
| Rmeas | 0.197 | 4.289 |
| Rpim | 0.062 | 1.639 |
| Number of reflections | 76674 | 3736 |
| <I/σ(I)> | 11.4 | 1.2 |
| Completeness [%] | 100.0 | 99.3 |
| Redundancy | 19.4 | 13.3 |
| CC(1/2) | 0.997 | 0.385 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M bis-tris, 14% (w/v) PEG 2000 MME, 12% (w/v) PEG 3350 |
