6I7S

Microsomal triglyceride transfer protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-21
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 1
Unit cell lengths	77.537, 105.595, 112.269
Unit cell angles	89.81, 76.95, 74.24

Refinement procedure

Resolution	49.350 - 2.500
R-factor	0.2071
Rwork	0.204
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lsh 4ekz
RMSD bond length	0.006
RMSD bond angle	1.356
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.350	49.350	2.650
High resolution limit [Å]	2.500	7.420	2.500
Rmerge	0.060	0.016	1.202
Rmeas	0.086	0.023	1.700
Number of reflections	108279	4043	16879
<I/σ(I)>	8.48	36.37	0.63
Completeness [%]	93.8	91.5	90.7
Redundancy	1.694	1.703	1.722
CC(1/2)	0.998	0.999	0.276

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.1 M Tris pH 8.25, 0.2 M Li2SO4, 32% v/v PEG 400, 2% Polypropylene glycol P425