6I5P
Co-crystal structure of human SPOP MATH domain (E47K) and human BRD3 fragment
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-23 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.9660 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.962, 90.334, 88.863 |
Unit cell angles | 90.00, 91.19, 90.00 |
Refinement procedure
Resolution | 19.931 - 1.810 |
R-factor | 0.1904 |
Rwork | 0.189 |
R-free | 0.22660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ivv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.053 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.170 | 1.754 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmeas | 0.058 | |
Number of reflections | 71635 | 11784 |
<I/σ(I)> | 11.82 | |
Completeness [%] | 98.6 | |
Redundancy | 3.23 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 200mM Ammonium Acetate, 100mM Tris pH 8.5, 25% (w/v) PEG 3350 |