6I5J

Crystal structure of SOCS2:Elongin C:Elongin B in complex with growth hormone receptor peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	P 2 21 21
Unit cell lengths	57.835, 113.707, 156.944
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.585 - 2.800
R-factor	0.2123
Rwork	0.210
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.412
Data scaling software	AutoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.080	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	26321
<I/σ(I)>	7.3
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	sodium cacodylate, cobalt chloride, MES, ammonium sulphate