6I34
Crystal structure of Neanderthal glycine decarboxylase (P-protein)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 86.873, 124.381, 201.304 |
Unit cell angles | 90.00, 98.57, 90.00 |
Refinement procedure
Resolution | 47.900 - 2.100 |
R-factor | 0.218 |
Rwork | 0.216 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6i33 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.915 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.900 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.192 | |
Number of reflections | 245944 | |
<I/σ(I)> | 5.3 | 1.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.1 | 5.2 |
CC(1/2) | 0.993 | 0.640 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.1 M HEPES pH 7, 6 % PEG3350, 10 mM DTT |