6I08
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT E243C-I201W
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 191 |
| Detector technology | PIXEL |
| Collection date | 2016-11-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.800, 134.350, 159.350 |
| Unit cell angles | 90.00, 101.83, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.120 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 3.060 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 73894 | |
| <I/σ(I)> | 12.5 | 1.6 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4 | 293 | PH 4, VAPOR DIFFUSION, TEMPERATURE 293K 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4. |
