6HZW

THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL 2.22 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	191
Detector technology	PIXEL
Collection date	2015-10-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9771
Spacegroup name	C 1 2 1
Unit cell lengths	182.416, 133.401, 160.474
Unit cell angles	90.00, 102.48, 90.00

Refinement procedure

Resolution	20.000 - 2.220
R-factor	0.1965
Rwork	0.195
R-free	0.22009
RMSD bond length	0.019
RMSD bond angle	2.234
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.390	2.260
High resolution limit [Å]	2.220	2.220
Number of reflections	169456
<I/σ(I)>	8.7
Completeness [%]	91.9
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4.