6HYR
THE GLIC PENTAMERIC LIGAND-GATED ION CHANNEL MUTANT Q193C+MMTS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-11-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9660 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.984, 128.285, 160.429 |
| Unit cell angles | 90.00, 100.96, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| R-factor | 0.13542 |
| Rwork | 0.231 |
| R-free | 0.24100 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.817 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.730 | 3.850 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Number of reflections | 39511 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 291 | 12-14.5% PEG4K; 15% GLycerol; 400 mM NaSCN; 3% DMSO; 100mM NaAcetate pH 4. |
