6HYQ

Regulatory subunit of a cAMP-independent protein kinase A from Trypanosoma cruzi bound to guanosine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	59.910, 80.819, 149.343
Unit cell angles	90.00, 92.80, 90.00

Refinement procedure

Resolution	59.839 - 2.080
R-factor	0.262630893136
Rwork	0.260
R-free	0.31914
RMSD bond length	0.019
RMSD bond angle	1.542
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.200
High resolution limit [Å]	2.080	2.080
Rmeas	0.134
Number of reflections	166708
<I/σ(I)>	7.64
Completeness [%]	98.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG 3350 (15 to 26%), Calcium acetate (0,05 to 0,4M), drop size: 500nL protein (10 mg/ml with 250 uM guanosine) +500nL buffer. Protein Purification Buffer (Size Exclusion Chromatography): 10mM Hepes pH 7.0, 50 mM NaCl, 1% DMSO, 1mM DTT, *The 6His tag was cleaved for crystallisation