6HYM

Structure of PCM1 LIR motif bound to GABARAP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9282
Spacegroup name	P 43
Unit cell lengths	80.873, 80.873, 55.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.587 - 1.860
R-factor	0.1871
Rwork	0.185
R-free	0.21750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gnu
RMSD bond length	0.019
RMSD bond angle	1.232
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.590	1.920
High resolution limit [Å]	1.860	1.860
Rmerge	0.020	0.780
Rmeas	0.020	0.890
Rpim	0.010	0.420
Number of reflections	30131	3045
<I/σ(I)>	19.7	1.3
Completeness [%]	99.5	97.7
Redundancy	4.7	4.3
CC(1/2)	1.000	0.650

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% w/v PEG 1500, 0.1 M SPG pH 8.5