6HYG
Heteromeric tandem IgG4/IgG1 Fc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.119, 63.253, 77.551 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 2.310 |
| R-factor | 0.25858 |
| Rwork | 0.258 |
| R-free | 0.27719 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3c2s |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.398 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.280 | 2.390 |
| High resolution limit [Å] | 2.310 | 2.310 |
| Number of reflections | 27962 | |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 95.7 | |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 5 mM zinc acetate, 100 mM sodium cacodylate pH 6.5, 9 % (w/v) polyethylene glycol 8000 |
