6HY3
Three-dimensional structure of AgaC from Zobellia galactanivorans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-03 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.604, 101.498, 46.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.420 - 1.300 |
| R-factor | 0.1503 |
| Rwork | 0.149 |
| R-free | 0.16892 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.944 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.420 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 70662 | |
| <I/σ(I)> | 11.66 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2.1 M sodium malonate and 1% glycerol |
