6HXR
Human PARP16 (ARTD15) IN COMPLEX WITH EB-47
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 148.064, 148.064, 99.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.700 - 2.900 |
| R-factor | 0.2009 |
| Rwork | 0.199 |
| R-free | 0.22970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f0d |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.223 | 2.250 |
| Number of reflections | 25189 | 3968 |
| <I/σ(I)> | 20.9 | 2.3 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 29.5 | 29.7 |
| CC(1/2) | 0.999 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 277 | 25% Poly(ethylene glycol) 3350, 0.2M ammonium acetate, 0.1M Bis-Tris, 1mM EB-47 |
