6HXF
Human STK10 bound to a maleimide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-17 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9195 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 94.840, 101.670, 198.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.452 - 2.090 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.21910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ajq |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.452 | 2.140 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.153 | |
| Rmeas | 0.166 | |
| Rpim | 0.064 | 0.638 |
| Number of reflections | 113868 | 8335 |
| <I/σ(I)> | 8.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.7 | 6.4 |
| CC(1/2) | 0.997 | 0.579 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.10M MgCl2; 0.1M TRIS pH 8.0; 20.0% PEG 6K; 10.0% EtGly |
