6HUT

GFP8 - a stabilized variant of cycle-3 GFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 21 21 21
Unit cell lengths	52.160, 63.370, 69.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.685 - 1.290
Rwork	0.186
R-free	0.20340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lqt
Phasing software	AMoRE (CCP4 7.0)
Refinement software	PHENIX (1.11.1-2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	1.490
High resolution limit [Å]	1.150	1.450
Rmeas		0.634
Number of reflections	58248	2865
<I/σ(I)>	20.2	2.6
Completeness [%]	99.6	95.3
Redundancy	7.9	7.9
CC(1/2)	1.000	0.200

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	296	200 mM HEPES, 10% v/v isopropanol, 200 mM NaCl