6HQ0

Crystal structure of ENL (MLLT1), apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 43 21 2
Unit cell lengths	49.419, 49.419, 128.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.180 - 1.810
R-factor	0.19369
Rwork	0.192
R-free	0.23661
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j9s
RMSD bond length	0.015
RMSD bond angle	1.459
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.120	1.840
High resolution limit [Å]	1.810	1.810
Rmerge	0.090	0.841
Rpim	0.028	0.286
Number of reflections	15436	895
<I/σ(I)>	14.3	3.2
Completeness [%]	100.0	99.8
Redundancy	11.9	10.2
CC(1/2)	0.998	0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	30% PEG 2000MME, 0.1M KBr