6HPT
Crystal structure of human Pif1 helicase, apoform.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.92819 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.540, 81.400, 91.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.890 - 1.440 |
| R-factor | 0.13692 |
| Rwork | 0.134 |
| R-free | 0.19468 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hph |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.452 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.400 | 1.480 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.045 | 1.329 |
| Number of reflections | 86350 | 6315 |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 6.6 | 6.8 |
| CC(1/2) | 0.999 | 0.514 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | Li2SO4, PEG 2K MME, MES |
