6HLM
Variant G129D of NuoEF from Aquifex aeolicus bound to NAD+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.541790 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.690, 63.293, 124.192 |
| Unit cell angles | 90.00, 107.18, 90.00 |
Refinement procedure
| Resolution | 51.490 - 1.800 |
| R-factor | 0.1774 |
| Rwork | 0.176 |
| R-free | 0.19740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.550 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.035 | 0.508 |
| Rpim | 0.037 | 0.509 |
| Number of reflections | 114854 | 5845 |
| <I/σ(I)> | 14.7 | 1.4 |
| Completeness [%] | 90.1 | 92.8 |
| Redundancy | 2.7 | 2.5 |
| CC(1/2) | 0.998 | 0.602 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 293 | 0.1M Tris/HCl 0.1M Sodium citrate |
