6HL7
Crystal structure of truncated aspartate transcarbamoylase from Plasmodium falciparum with mutated active site (R109A/K138A) and N-carbamoyl-L-phosphate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-04-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.001, 89.715, 121.642 |
| Unit cell angles | 90.00, 122.17, 90.00 |
Refinement procedure
| Resolution | 57.610 - 2.500 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ilq |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.946 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.610 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmeas | 0.066 | 0.957 |
| Number of reflections | 46404 | 7154 |
| <I/σ(I)> | 12.95 | |
| Completeness [%] | 97.6 | 94 |
| Redundancy | 3.16 | 3.21 |
| CC(1/2) | 0.999 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M Potassium citrate tribasic monohydrate 20% w/v PEG 3350 |
