6HKW
Crystal structure of human SDS22
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.764, 81.153, 191.958 |
| Unit cell angles | 90.00, 104.37, 90.00 |
Refinement procedure
| Resolution | 46.863 - 3.090 |
| R-factor | 0.2112 |
| Rwork | 0.210 |
| R-free | 0.23880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4aw4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.021 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.790 | 3.130 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.160 | 0.733 |
| Number of reflections | 94224 | |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | ammonium sulfate, PEG 4000, glycerol |
