6HKP

Factor Inhibiting HIF (FIH) in complex with zinc, NOG and ASPP2 (970-992)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9793
Spacegroup name	P 41 21 2
Unit cell lengths	86.020, 86.020, 148.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.287 - 1.900
R-factor	0.1765
Rwork	0.175
R-free	0.19480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.005
RMSD bond angle	0.684
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.020	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.180	1.923
Rmeas	0.186	1.987
Rpim	0.046	0.496
Number of reflections	44748	6404
<I/σ(I)>	8.3
Completeness [%]	100.0	100
Redundancy	16	15.6
CC(1/2)	0.992	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M Hepes pH = 7.5, 2.0 M ammonium sulfate, 5.5% PEG400