6HK6
Human RIOK2 bound to inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-08 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9201 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 92.010, 92.860, 237.650 |
| Unit cell angles | 90.00, 99.10, 90.00 |
Refinement procedure
| Resolution | 78.340 - 2.350 |
| R-factor | 0.21595 |
| Rwork | 0.215 |
| R-free | 0.23543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gyi |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.415 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.860 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.110 | 0.590 |
| Number of reflections | 164333 | 8004 |
| <I/σ(I)> | 6.6 | 1.8 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 3.1 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 10% ethylene glycol, 0.1 M bis-tris-propane pH 6.5 and 0.2 M sodium/potassium tartrat |
