6HJY
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) Delta8 truncation mutant in complex with nanobody 72
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-24 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 0.9754 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 95.675, 155.093, 101.618 |
| Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
| Resolution | 49.490 - 2.780 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.23300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6hjx |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.490 | 2.850 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.098 | 1.442 |
| Rmeas | 0.136 | 1.998 |
| Rpim | 0.094 | 1.379 |
| Number of reflections | 71824 | 4318 |
| <I/σ(I)> | 6.6 | 0.6 |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.995 | 0.281 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 300 mM ammonium formate, 50 mM TRIS pH 9.0 and 33% PEG monomethylether 550 |
