6HJY

X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) Delta8 truncation mutant in complex with nanobody 72

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-24
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.9754
Spacegroup name	P 1 21 1
Unit cell lengths	95.675, 155.093, 101.618
Unit cell angles	90.00, 103.08, 90.00

Refinement procedure

Resolution	49.490 - 2.780
R-factor	0.226
Rwork	0.225
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hjx
RMSD bond length	0.008
RMSD bond angle	1.030
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.490	2.850
High resolution limit [Å]	2.780	2.780
Rmerge	0.098	1.442
Rmeas	0.136	1.998
Rpim	0.094	1.379
Number of reflections	71824	4318
<I/σ(I)>	6.6	0.6
Completeness [%]	99.3
Redundancy	3.3
CC(1/2)	0.995	0.281

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	300 mM ammonium formate, 50 mM TRIS pH 9.0 and 33% PEG monomethylether 550