6HHD
Mouse Prion Protein in complex with Nanobody 484
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-11-03 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.130, 63.798, 69.792 |
| Unit cell angles | 90.00, 101.96, 90.00 |
Refinement procedure
| Resolution | 57.846 - 2.102 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ma7 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.550 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.850 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 29594 | |
| <I/σ(I)> | 10.3 | |
| Completeness [%] | 99.5 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.15 M ammonium sulfate, 0.1 M MES pH6.0, 15% w/v PEG 40000. |
