6HGO

Crystal Structure of human IL-17F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.99985
Spacegroup name	P 65
Unit cell lengths	126.195, 126.195, 88.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.100 - 2.100
R-factor	0.2
Rwork	0.199
R-free	0.22000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1jpy
RMSD bond length	0.010
RMSD bond angle	1.120
Data reduction software	XDS (Sept. 26, 2012)
Data scaling software	XSCALE (July 4, 2012)
Phasing software	BUSTER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.100	63.100	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.078	0.023	1.413
Rmeas	0.082	0.024	1.488
Total number of observations	477335
Number of reflections	46829	559	3421
<I/σ(I)>	18.6	64.33	1.66
Completeness [%]	100.0	99.6	100
Redundancy	10.193	10.075	10.117
CC(1/2)	0.999	0.999	0.603

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	200mM ammonium sulfate, 20.0% PEG 3350