6HGA

Crystal Structure of the human IL-17RC D2-D3-D4 domains in complex with an anti-APP tag Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-08
Detector	PSI PILATUS 6M
Wavelength(s)	0.99998
Spacegroup name	P 62 2 2
Unit cell lengths	250.910, 250.910, 78.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	82.130 - 2.600
R-factor	0.206
Rwork	0.205
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS (Oct. 15, 2015)
Data scaling software	XSCALE (Oct. 15, 2015)
Phasing software	PHASER
Refinement software	BUSTER (2.11.7)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	82.130	82.130	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.168	0.025	3.364
Rmeas	0.173	0.026	3.458
Number of reflections	45204	608	3300
<I/σ(I)>	17.99	94.66	1
Completeness [%]	100.0	99.2	100
Redundancy	19.973	17.84	18.748
CC(1/2)	0.999	1.000	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	292	0.1M HEPES, 30% Jeffamine ED-2001