6HG9

Crystal Structure of the human IL-17RC ECD in complex with human IL-17F, Crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-18
Detector	PSI PILATUS 6M
Wavelength(s)	0.99996
Spacegroup name	H 3 2
Unit cell lengths	160.312, 160.312, 197.983
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	56.836 - 3.620
R-factor	0.2272
Rwork	0.226
R-free	0.25770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jpy
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.600	80.600	3.820
High resolution limit [Å]	3.620	11.450	3.620
Rmerge	0.099	0.024	2.146
Rmeas	0.105	0.025	2.255
Rpim	0.033	0.008	0.689
Number of reflections	11380	397	1642
<I/σ(I)>	16.9
Completeness [%]	100.0	99.6	99.9
Redundancy	10.2	8.8	10.6
CC(1/2)	1.000	1.000	0.515

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	200mM ammonium formate, 20.0% PEG 3350