6HG4

Crystal Structure of the human IL-17RC ECD in complex with human IL-17F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-12
Detector	PSI PILATUS 6M
Wavelength(s)	0.99994
Spacegroup name	P 3 2 1
Unit cell lengths	154.322, 154.322, 78.792
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.790 - 3.320
R-factor	0.2
Rwork	0.198
R-free	0.23900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jpy
RMSD bond length	0.010
RMSD bond angle	1.260
Data reduction software	XDS (Autoproc)
Data scaling software	Aimless (0.7.1)
Phasing software	BUSTER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	133.660	133.660	3.500
High resolution limit [Å]	3.320	10.490	3.320
Rmerge	0.181	0.051	3.757
Rmeas	0.185	0.053	3.865
Rpim	0.038	0.012	0.899
Number of reflections	16301	571	2348
<I/σ(I)>	18
Completeness [%]	100.0	99.9	100
Redundancy	23.5	21.5	18.2
CC(1/2)	0.999	0.999	0.454

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	200mM ammonium citrate tribasic, 20.0% PEG 3350