6HFB

Outward-facing conformation of a multidrug resistance MATE family transporter of the MOP superfamily.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2008-12-16
Detector	PSI PILATUS 6M
Wavelength(s)	1.2
Spacegroup name	C 1 2 1
Unit cell lengths	219.752, 94.246, 138.785
Unit cell angles	90.00, 126.48, 90.00

Refinement procedure

Resolution	19.999 - 3.495
R-factor	0.1915
Rwork	0.189
R-free	0.23920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mlb
RMSD bond length	0.002
RMSD bond angle	0.415
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.130	3.620
High resolution limit [Å]	3.495	3.495
Rmerge	0.090	0.318
Rmeas	0.106	0.370
Rpim	0.054	0.188
Number of reflections	54531	2738
<I/σ(I)>	7.3	5
Completeness [%]	99.3	95.5
Redundancy	3.8	3.7
CC(1/2)	0.995	0.908

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	30% PEG 2000 MME, 0.1 M ADA, pH 6.5, 0.15% octyl-beta-D-selenoglucoside; crystal soaking in 1 M CsCl