6HD9
Crystal structure of the potassium channel MtTMEM175 with rubidium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.79480 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 130.480, 130.480, 132.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.500 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmeas | 0.279 | 3.512 |
Rpim | 0.033 | 0.740 |
Number of reflections | 14836 | 1400 |
<I/σ(I)> | 11.4 | 1.4 |
Completeness [%] | 99.3 | 93.2 |
Redundancy | 27.7 | 23 |
CC(1/2) | 1.000 | 0.511 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Tris-HCl pH 8.5, 150 mM NaCl, 150 mM MgCl2 and 28- 30% PEG400 |