6HD0

Common mode of remodeling AAA ATPases p97/CDC48 by their disassembly cofactors ASPL/PUX1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91841
Spacegroup name	P 31
Unit cell lengths	185.120, 185.120, 122.426
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.980 - 3.728
R-factor	0.2117
Rwork	0.208
R-free	0.25290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s3s 5ifs
RMSD bond length	0.002
RMSD bond angle	1.168
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.980	4.030
High resolution limit [Å]	3.728	3.880
Rmerge	0.306
Rmeas	0.340	0.269
Rpim	0.148
Number of reflections	28980	668
<I/σ(I)>	6.2	0.75
Completeness [%]	90.7	98.1
Redundancy	5.3
CC(1/2)	0.983	0.324

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	6% (v/v) PEG1500, 0.2M sodium acetate, 0.1M Hepes pH 7.0