6HC2
Crystal structure of NuMA/LGN hetero-hexamers
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-25 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9698 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 153.940, 153.940, 732.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 150.653 - 4.310 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.23410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdb entries 3SF4 and 3RO2 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.003 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 243.480 | 4.400 |
| High resolution limit [Å] | 4.290 | 4.290 |
| Rmerge | 0.343 | 7.028 |
| Number of reflections | 61354 | |
| <I/σ(I)> | 19.1 | 2.1 |
| Completeness [%] | 99.9 | 98.9 |
| Redundancy | 98.5 | 88.7 |
| CC(1/2) | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 10% PEG3350, 0.05M Bis-Tris propane and 0.1M Na-formate or 0.1M Na-acetate trihydrate |
