6H6K
The structure of the FKR mutant of the archaeal translation initiation factor 2 gamma subunit in complex with GDPCP, obtained in the absence of magnesium salts in the crystallization solution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 193.15 |
| Detector technology | CCD |
| Collection date | 2010-05-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 1 |
| Unit cell lengths | 76.710, 76.750, 146.790 |
| Unit cell angles | 101.46, 92.08, 95.97 |
Refinement procedure
| Resolution | 20.030 - 2.000 |
| R-factor | 0.2173 |
| Rwork | 0.216 |
| R-free | 0.24780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rjl |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.585 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.030 | 2.000 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Number of reflections | 235926 | 20932 |
| <I/σ(I)> | 7.53 | 0.66 |
| Completeness [%] | 93.1 | 62.9 |
| Redundancy | 1.84 | 1.17 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 301.15 | Sodium chloride, 2-mercaptoethanol, Tris-HCl, sodium malonate, GDPCP, ethylene glycol. |
