6H62

QTRT1, the catalytic subunit of murine tRNA-Guanine Transglycosylase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.282814
Spacegroup name	P 21 21 21
Unit cell lengths	83.704, 93.767, 117.127
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.985 - 2.684
R-factor	0.1886
Rwork	0.187
R-free	0.22760
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	3.630	2.850
High resolution limit [Å]	2.680	3.420	2.680
Number of reflections	49608	3719	7918
<I/σ(I)>	13.95	3.7	3
Completeness [%]	99.7
Redundancy	6.98	6.9	6.98

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	291.15	100mM CAPS pH 10.5, 200 mM Sodium chloride, 20% PEG 8000
1	VAPOR DIFFUSION, SITTING DROP	10.5	291.15	100mM CAPS pH 10.5, 200 mM Sodium chloride, 20% PEG 8000